Down-regulation of GATA-3 expression during human papillomavirus-mediated immortalization and cervical carcinogenesis.

ABSTRACT: To identify cellular genes that may be involved in human papillomavirus (HPV)-mediated immortalization mRNA differential display analysis was performed on preimmortal and subsequent immortal stages of four human keratinocyte cell lines transformed by HPV type 16 or 18 DNA. This yielded a cDNA fragment encoding the transcription factor GATA-3 that was strongly reduced in intensity in all immortal stages of the four cell lines. A marked reduction in both GATA-3 mRNA and protein expression in HPV-immortalized cell lines was confirmed by reverse transcriptase-polymerase chain reaction, Western blot analysis, and immunohistochemistry and was also shown to be apparent in cervical carcinoma cell lines. Immunohistochemical analysis of cervical tissue specimens showed a clear nuclear staining for G

SUBMITTER: Steenbergen RD 

PROVIDER: S-EPMC1850837 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature