Roles for IL-1 and TNFalpha in dynamic behavioral responses of Langerhans cells to topical hapten application.

ABSTRACT:

Background

To understand the behavioral biology of Langerhans cells (LCs), we recently recorded time-lapse images of LCs in the knock-in mice expressing the I-Abeta chain tagged with the enhanced green fluorescence protein (EGFP). EGFP(+) LCs showed relatively limited motility in the steady state, whereas topical application of dinitrofluorobenzene (DNFB) markedly augmented a unique movement of dendrites characterized by rhythmic extension and retraction, termed dSEARCH, and triggered amoeba-like lateral migration of cell bodies.

Objective

To define underlying mechanisms by which hapten treatment alters LC behaviors.

Methods

The I-Abeta-EGFP mice received subcutaneous (s.c.) injection of recombinant IL-1alpha or TNFalpha (50 ng/animal) and dynamic behaviors of EGFP(+)

SUBMITTER: Nishibu A 

PROVIDER: S-EPMC1850973 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature