Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production.

ABSTRACT: An increased dependency on glycolysis for ATP production is considered to be a hallmark of tumor cells. Whether this increase in glycolytic activity is due mainly to inherent metabolic alterations or to the hypoxic microenvironment remains controversial. Here we have transformed human adult mesenchymal stem cells (MSC) using genetic alterations as described for differentiated cells. Our data suggest that MSC require disruption of the same pathways as have been shown for differentiated cells to confer a fully transformed phenotype. Furthermore, we found that MSC are more glycolytic than primary human fibroblasts and, in contrast to differentiated cells, do not depend on increased aerobic glycolysis for ATP production during transformation. These data indicate that aerobic glycolysis (the Wa

SUBMITTER: Funes JM 

PROVIDER: S-EPMC1851087 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature