Meiotic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation.

ABSTRACT: Meiotic recombination-related DNA synthesis (MRDS) was analyzed in Saccharomyces cerevisiae by specifically timed incorporation of thymidine analogs into chromosomes. Lengths and positions of incorporation tracts were determined relative to a known recombination hot spot along DNA, as was the timing and localization of incorporation relative to forming and formed synaptonemal complex in spread chromosomes. Distinct patterns could be specifically associated with the majority cross-over and non-cross-over recombination processes. The results obtained provide direct evidence for key aspects of current consensus recombination models, provide information regarding temporal and spatial relationships between non-cross-over formation and the synaptonemal complex, and raise the possibility that rem

SUBMITTER: Terasawa M 

PROVIDER: S-EPMC1851600 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature