Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method.

ABSTRACT: Recent genome-wide transcriptome analysis has identified diverse classes of non-coding RNAs (ncRNAs), some of which have been demonstrated to be functional, regulatory RNAs involved in various biological processes. Maturation of RNA molecules through various post-transcriptional processing events, including splicing, modification, editing and trimming of both ends, is required for correct folding and proper function of RNA molecules. To characterize post-transcriptional modifications and terminal chemical structures of fully processed native RNAs, it is necessary to isolate individual RNA species from a limited quantity and complex mixture of cellular RNAs. However, there have been no general and convenient strategies for isolation of individual RNAs. We describe here the first example of

SUBMITTER: Miyauchi K 

PROVIDER: S-EPMC1851638 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature