Multiple-pathway analysis of double-strand break repair mutations in Drosophila.

ABSTRACT: The analysis of double-strand break (DSB) repair is complicated by the existence of several pathways utilizing a large number of genes. Moreover, many of these genes have been shown to have multiple roles in DSB repair. To address this complexity we used a repair reporter construct designed to measure multiple repair outcomes simultaneously. This approach provides estimates of the relative usage of several DSB repair pathways in the premeiotic male germline of Drosophila. We applied this system to mutations at each of 11 repair loci plus various double mutants and altered dosage genotypes. Most of the mutants were found to suppress one of the pathways with a compensating increase in one or more of the others. Perhaps surprisingly, none of the single mutants suppressed more than one pathway

SUBMITTER: Johnson-Schlitz DM 

PROVIDER: S-EPMC1851981 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature