Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis.

ABSTRACT:

Background

Invasive ductal and lobular carcinomas (IDC and ILC) are the most common histological types of breast cancer. Clinical follow-up data and metastatic patterns suggest that the development and progression of these tumors are different. The aim of our study was to identify gene expression profiles of IDC and ILC in relation to normal breast epithelial cells.

Methods

We examined 30 samples (normal ductal and lobular cells from 10 patients, IDC cells from 5 patients, ILC cells from 5 patients) microdissected from cryosections of ten mastectomy specimens from postmenopausal patients. Fifty nanograms of total RNA were amplified and labeled by PCR and in vitro transcription. Samples were analysed upon Affymetrix U133 Plus 2.0 Arrays. The expression of seven differentially

SUBMITTER: Turashvili G 

PROVIDER: S-EPMC1852112 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature