A focus reduction neutralization assay for hepatitis C virus neutralizing antibodies.

ABSTRACT:

Background/aim

The role of humoral immunity in hepatitis C virus (HCV) infection is poorly understood. Nevertheless, there is increasing interest in characterizing the neutralizing antibodies in the serum of HCV-infected patients. Focus reduction assays have been widely used to evaluate neutralizing antibody responses against a range of non-cytopathic viruses. Based on the recent development of a HCV cell culture system using the genotype 2 JFH-1-strain, we developed a focus reduction assay for HCV-neutralizing antibodies.

Methods

The focus reduction assay was based on a standard microneutralization assay in which immunostained foci on tissue culture plates are counted. The neutralizing anti-HCV antibodies titers of purified serum immunoglobulin samples from seventy-seven in

SUBMITTER: Fournier C 

PROVIDER: S-EPMC1852297 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature