Long-range chromosomal interactions regulate the timing of the transition between poised and active gene expression.

ABSTRACT: To understand how mammalian genes are regulated from their natural chromosomal environment, we have analysed the molecular events occurring throughout a 150 kb chromatin segment containing the alpha globin gene locus as it changes from a poised, silent state in erythroid progenitors, to the fully activated state in late, erythroid cells. Active transcription requires the late recruitment of general transcription factors, mediator and Pol II not only to the promoter but also to its remote regulatory elements. Natural mutants of the alpha cluster show that whereas recruitment of the pre-initiation complex to the upstream elements occurs independently, recruitment to the promoter is largely dependent on the regulatory elements. An improved, quantitative chromosome conformation capture analysi

SUBMITTER: Vernimmen D 

PROVIDER: S-EPMC1852780 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature