Telomerase activity and expression of telomerase RNA component and telomerase catalytic subunit gene in cervical cancer.

ABSTRACT: Telomerase, a ribonucleoprotein complex that includes the telomerase RNA component (hTR) and the telomerase catalytic subunit gene (hTERT) product, has been shown to be activated in the majority of cancer tissues and immortalized cells. To study telomerase activation during the progression of cervical cancer, the expression of hTR and hTERT RNAs in tissues of various stages of cervical cancer was analyzed using the in situ hybridization method and compared with proliferative activity as estimated by Ki-67 immunostaining. To test whether expression of these components is reflected in enzyme activity, we determined the levels of the RNAs in cervical cancer and normal tissues and in primary and immortal keratinocytes by reverse transcription-polymerase chain reaction and RNase protection assa

SUBMITTER: Nakano K 

PROVIDER: S-EPMC1853016 | biostudies-literature | 1998 Sep

REPOSITORIES: biostudies-literature