Global transcriptional analysis delineates the differential inflammatory response interleukin-15 elicits from cultured human T cells.

ABSTRACT:

Objective

Interleukin 15 (IL)-15 controls proliferation and survival of T cells, but its effects and the underlying cellular regulation are not well understood. Previous studies have focused on its effects on short-term T-cell cultures. In view of the potential problems associated with using IL-2 alone in adoptive immunotherapy protocols, we investigated the impact of IL-15 on T-cell cultures and the global transcriptional effects it elicits in such cultures.

Materials and methods

DNA microarrays and flow cytometry were used to examine the differential effect of 20 ng/mL IL-15 on primary serum-free T-cell cultures activated and cultured in the presence of IL-2. Quantitative reverse transcriptase polymerase chain reaction confirmed select microarray data.

Results

IL-15

SUBMITTER: Ramsborg CG 

PROVIDER: S-EPMC1855244 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature