Human prostate epithelium lacks Wee1A-mediated DNA damage-induced checkpoint enforcement.

ABSTRACT: Cellular DNA damage triggers the DNA damage response pathway and leads to enforcement of cell cycle checkpoints, which are essential for the maintenance of genomic integrity and are activated in early stages of tumorigenesis. A special feature of prostate cancer is its high incidence and multifocality. To address the functionality of DNA damage checkpoints in the prostate, we analyzed the responses of human primary prostate epithelial cells (HPECs) and freshly isolated human prostate tissues to gamma-irradiation. We find that gamma-irradiation activates the ataxia telangiectasia mutated-associated DNA damage response pathway in the HPECs but that the clearance of phosphorylated histone H2AX (gammaH2AX) foci is delayed. Surprisingly, gamma-irradiated HPECs were unable to enforce cell cycle

SUBMITTER: Kiviharju-af Hallstrom TM 

PROVIDER: S-EPMC1855358 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature