Gene expression in vivo shows that Helicobacter pylori colonizes an acidic niche on the gastric surface.

ABSTRACT: Helicobacter pylori is a gastric-dwelling pathogen responsible, with acid secretion, for peptic ulcer and a 20-fold increase in the risk of gastric cancer. Several transcriptomes have been described after short-term exposure to acidity in vitro, but there are no data identifying the effects of chronic gastric exposure on bacterial gene expression. Comparison of the in vivo to the in vitro transcriptome at pH 7.4 identified several groups of genes of known function that increased expression >2-fold, and three of these respond both to acidity in vitro and to gastric infection. Almost all known acid acclimation genes are highly up-regulated. These include ureA, ureB, and rocF and the pH-gated urea channel, ureI. There is also up-regulation of two groups of motility and chemotaxis genes and fo

SUBMITTER: Scott DR 

PROVIDER: S-EPMC1855417 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature