Green autofluorescence in dinoflagellates, diatoms, and other microalgae and its implications for vital staining and morphological studies.

ABSTRACT: Green autofluorescence (GAF) has been described in the short flagellum of golden and brown algae, the stigma of Euglenophyceae, and cytoplasm of different life stages of dinoflagellates and is considered by some researchers a valuable taxonomic feature for dinoflagellates. In addition, green fluorescence staining has been widely proposed or adopted to measure cell viability (or physiological state) in areas such as apoptosis of phytoplankton, pollutant stresses on algae, metabolic activity of algae, and testing treatment technologies for ships' ballast water. This paper reports our epifluorescence microscopic observations and quantitative spectrometric measurements of GAF in a broad phylogenetic range of microalgae. Our results demonstrate GAF is a common feature of dinoflagellates, diatom

SUBMITTER: Tang YZ 

PROVIDER: S-EPMC1855647 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature