Molecular fingerprinting of the fecal microbiota of children raised according to different lifestyles.

ABSTRACT: In this population-based study, 90 children from three European countries were examined to determine the impact of lifestyle on the fecal microbiota. The study was designed to assess the impact of two extreme lifestyles that we hypothesized could impact the microbial composition in the gut: i.e., an anthroposophic lifestyle (restricted use of antibiotics, greater consumption of fermented vegetables, etc.) versus living on a farm (greater consumption of farm milk, contact with animals, etc.). In previous studies, these lifestyles correlated with lower prevalence of allergies. Terminal restriction fragment length polymorphism (T-RFLP) was used to assess the bacterial composition in fecal samples since recent studies have shown that the majority of this community cannot be cultivated. The T-R

SUBMITTER: Dicksved J 

PROVIDER: S-EPMC1855685 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature