Regulatory role of PopN and its interacting partners in type III secretion of Pseudomonas aeruginosa.

ABSTRACT: The type III secretion system (T3SS) of Pseudomonas aeruginosa plays a significant role in pathogenesis. We have previously identified type III secretion factor (TSF), which is required for effective secretion of the type III effector molecules, in addition to the low calcium signal. TSF includes many low-affinity high-capacity calcium binding proteins, such as serum albumin and casein. A search for the TSF binding targets on the bacterial outer membrane resulted in identification of PopN, a component of the T3SS that is readily detectable on the bacterial cell surface. PopN specifically interacts with Pcr1, and both popN and pcr1 mutants have a constitutive type III secretion phenotype, suggesting that the two proteins form a complex that functions as a T3SS repressor. Further analysis of

SUBMITTER: Yang H 

PROVIDER: S-EPMC1855783 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature