Molecular characterisation of non-absorptive and absorptive enterocytes in human small intestine.

ABSTRACT:

Background and aims

Perturbation of differentiation of the crypt-villus axis of the human small intestine is associated with several intestinal disorders of clinical importance. At present, differentiation of small intestinal enterocytes in the crypt-villus axis is not well characterised.

Subjects and methods

Expression profiling of microdissected enterocytes lining the upper part of crypts or the middle of villi was performed using the Affymetrix X3P arrays and several methods for confirmation.

Results

A total of 978 differentially expressed sequences representing 778 unique UniGene IDs were found and categorised into four functional groups. In enterocytes lining the upper part of crypts, cell cycle promoting genes and transcription/translation related genes were pre

SUBMITTER: Gassler N 

PROVIDER: S-EPMC1856251 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature