Global analysis of the human gastric epithelial transcriptome altered by Helicobacter pylori eradication in vivo.

ABSTRACT:

Objective

The transcriptional profile of gastric epithelial cell lines cocultured with Helicobacter pylori and the global gene expression of whole gastric mucosa has been described previously. We aimed to overcome limitations of previous studies by determining the effects of H pylori eradication on the transcriptome of purified human gastric epithelium using each patient as their own control.

Design

Laser capture microdissection (LCM) was used to extract mRNA from paraffin-embedded antral epithelium from 10 patients with peptic ulcer disease, before and after H pylori eradication. mRNA was reverse transcribed and applied on to Affymetrix cDNA microarray chips customised for formalin-fixed tissue. Differentially expressed genes were identified and a subset validated by real-t

SUBMITTER: Resnick MB 

PROVIDER: S-EPMC1856477 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature