Proteomic analysis of human meibomian gland secretions.

ABSTRACT:

Background/aim

Human tears contain hundreds of proteins that may exert a significant influence on tear film stability, ocular surface integrity, and visual function. The authors hypothesise that many of these proteins originate from the meibomian gland. This study's aim was to begin to develop the proteomic methodology to permit the testing of their hypothesis.

Methods

Meibomian gland secretions were collected from the lower eyelids of adult volunteers and placed in a chloroform-methanol mixture. Samples were partitioned in a biphasic system and non-lipid phase materials were reduced, alkylated, and trypsin digested to obtain peptides for protein identification. This peptide mixture was separated by micro-capillary reverse phase chromatography and the effluent examined by na

SUBMITTER: Tsai PS 

PROVIDER: S-EPMC1856970 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature