Expression of insulin-like growth factor binding protein-3 in pterygium tissue.

ABSTRACT:

Background

Pterygium is a fibrovascular overgrowth from the conjunctiva onto the cornea. The pathogenesis of this common ocular surface disorder is not well understood and the only treatment is surgical removal.

Methods

DNA microarray analysis of primary pterygium tissue was carried out using uninvolved conjunctiva tissue as a comparison for gene expression levels. Real time polymerase chain reaction (PCR) was used to verify the mRNA level of expression for genes changed in pterygium. Western blot analysis and immunohistochemistry showed protein expression levels and the tissue distribution.

Results

Microarray analysis revealed that mRNA levels of a number of genes were changed in primary pterygium. In particular the gene for insulin-like growth factor binding protein

SUBMITTER: Wong YW 

PROVIDER: S-EPMC1860207 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature