Caspase activity is downregulated in choriocarcinoma: a cDNA array differential expression study.

ABSTRACT:

Background

Placental trophoblast can be considered to be pseudomalignant tissue and the pathogenesis of gestational trophoblastic diseases remains to be clarified.

Aims

To examine the role of caspases 8 and 10, identified by differential expression, on trophoblast tumorigenesis.

Methods

cDNA array hybridisation was used to compare gene expression profiles in choriocarcinoma cell lines (JAR, JEG, and BeWo) and normal first trimester human placentas, followed by confirmation with quantitative real time polymerase chain reaction and immunohistochemistry. Caspase 10 and its closely related family member caspase 8 were analysed.

Results

Downregulation of caspase 10 in choriocarcinoma was detected by both Atlastrade mark human cDNA expression array and Atlastrade mar

SUBMITTER: Fong PY 

PROVIDER: S-EPMC1860314 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature