Pitfalls in immunohistochemical assessment of EGFR expression in soft tissue sarcomas.

ABSTRACT:

Background

New targeted cancer treatments acting against growth factor receptors such as the epidermal growth factor receptor (EGFR) necessitate selecting patients for treatment with these drugs. Besides carcinomas, soft tissue sarcomas (STS) express EGFR and might thereby be a promising target for this new therapeutic strategy.

Objective

To test and compare different EGFR antibodies to determine the frequency of EGFR expression in STS.

Methods

302 consecutive specimens of STS were examined using the tissue microarray technique. EGFR expression levels were assessed by immunohistochemistry using five different commercially available antibodies. Gene amplification status was measured by fluorescence in situ hybridisation (FISH). Immunoreactivity and amplification status

SUBMITTER: Kersting C 

PROVIDER: S-EPMC1860383 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature