Photophysics of Clomeleon by FLIM: discriminating excited state reactions along neuronal development.

ABSTRACT: In this work, fluorescence lifetime imaging microscopy in the time domain was used to study the fluorescence dynamics of ECFP and of the ratiometric chloride sensor Clomeleon along neuronal development. The multiexponential analysis of fluorophores combined with the study of the contributions of the individual lifetimes (decay-associated spectra) was used to discriminate the presence of energy transfer from other excited state reactions. A characteristic change of sign of the pre-exponential factors of lifetimes from positive to negative near the acceptor emission maxima was observed in presence of energy transfer. By fluorescence lifetime imaging microscopy, we could show that the individual conformations of CFP display differential quenching properties depending on their microenvironment

SUBMITTER: Jose M 

PROVIDER: S-EPMC1861805 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature