Evolutionary proteomics identifies amino acids essential for ligand-binding of the cytokinin receptor CHASE domain.

ABSTRACT:

Background

In plants the hormone cytokinin is perceived by members of a small cytokinin receptor family, which are hybrid sensor histidine kinases. While the immediate downstream signaling pathway is well characterized, the domain of the receptor responsible for ligand binding and which residues are involved in this process has not been determined experimentally.

Results

Using a live cell hormone-binding assay, we show that cytokinin is bound by a receptor domain predicted to be extracellular, the so called CHASE (cyclases, histidine kinase associated sensory extracellular) domain. The CHASE domain occurs not only in plant cytokinin receptors but also in numerous orphan receptors in lower eukaryotes and bacteria. Taking advantage of this fact, we used an evolutionary proteom

SUBMITTER: Heyl A 

PROVIDER: S-EPMC1863423 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature