Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.

ABSTRACT: Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.

SUBMITTER: Bialasiewicz S 

PROVIDER: S-EPMC1865911 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature