Interaction of rotavirus polymerase VP1 with nonstructural protein NSP5 is stronger than that with NSP2.

ABSTRACT: Rotavirus morphogenesis starts in intracellular inclusion bodies called viroplasms. RNA replication and packaging are mediated by several viral proteins, of which VP1, the RNA-dependent RNA polymerase, and VP2, the core scaffolding protein, were shown to be sufficient to provide replicase activity in vitro. In vivo, however, viral replication complexes also contain the nonstructural proteins NSP2 and NSP5, which were shown to be essential for replication, to interact with each other, and to form viroplasm-like structures (VLS) when coexpressed in uninfected cells. In order to gain a better understanding of the intermediates formed during viral replication, this work focused on the interactions of NSP5 with VP1, VP2, and NSP2. We demonstrated a strong interaction of VP1 with NSP5 but only a

SUBMITTER: Arnoldi F 

PROVIDER: S-EPMC1865955 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature