Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.

ABSTRACT: We have used Affymetrix high-density gene arrays to generate a temporal profile of gene expression during differentiation of UB/OC-1, a conditionally immortal cell line derived from the mouse cochlea. Gene expression was assessed daily for 14 days under differentiating conditions. The experiment was replicated in two separate populations of cells. Profiles for selected genes were correlated with those obtained by RT-PCR, TaqMan analysis, immunoblotting, and immunofluorescence. The results suggest that UB/OC-1 is derived from a population of nonsensory epithelial cells in the greater epithelial ridge that have the potential to differentiate into a hair-cell-like phenotype, without the intervention of Math1. Elements of the Notch signaling cascade were identified, including the receptor Notc

SUBMITTER: Rivolta MN 

PROVIDER: S-EPMC186616 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature