S100A1 and S100B, transcriptional targets of SOX trio, inhibit terminal differentiation of chondrocytes.

ABSTRACT: Transcription factor SOX9 (sex-determining region Y-type high mobility group box 9) and its coactivators SOX5 and SOX6 (the SOX trio) induce early-stage chondrocyte differentiation and suppress its terminal stage. To identify possible targets of the SOX trio, we carried out a microarray analysis and identified S100A1 and S100B as possible target molecules. S100 protein expression was localized in late proliferative and pre-hypertrophic chondrocytes of the mouse growth plate. Overexpression of S100A1, S100B or their combination in cultured chondrogenic cells did not induce early differentiation, but suppressed hypertrophic differentiation and mineralization. Silencing of both S100A1 and S100B stimulated terminal differentiation and reversed the SOX-trio-mediated inhibition. Finally, lucifer

SUBMITTER: Saito T 

PROVIDER: S-EPMC1866207 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature