Single nucleotide polymorphism profiling assay to confirm the identity of human tissues.

ABSTRACT: To identify issues of sample mix-ups, various molecular techniques are currently used. These techniques, however, are time consuming and require experience and/or DNA sequencing equipment or have a relatively high risk of errors because of contamination. Therefore, a quick and straightforward single nucleotide polymorphism (SNP) profiling assay was developed to link human tissues to a source. SNPs are common sequence variations in the human genome, and each individual has a unique combination of these nucleotide variations. Using potentially mislabeled paraffin-embedded tissues, DNA was extracted and SNP profiles were determined by real-time polymerase chain reaction analysis of the purified DNA using a selection of 10 commercially available SNP amplification assays. These profiles were co

SUBMITTER: Huijsmans R 

PROVIDER: S-EPMC1867440 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature