Establishment and study of different real-time polymerase chain reaction assays for the quantification of cells with deletions of chromosome 7.

ABSTRACT: The evaluation of residual disease, which has prognostic value in the treatment of hematological malignancies, is currently assessed by scoring a limited number of cells by karyotyping and molecular cytogenetics. Real-time polymerase chain reaction (PCR) is an easier and more sensitive technique, enables analysis of a larger number of cells, and decreases sampling error. However, real-time PCR has been applied only to target transcripts of fusion genes. Here, we considered two real-time PCR strategies to quantify a number of cells carrying a partial deletion of chromosome 7 mixed with normal disomic cells. The first strategy was based on the amplification of two sequences, one on chromosome 7 and the other on chromosome 14. In the second strategy residual disease was assessed by the ratio

SUBMITTER: Mattarucchi E 

PROVIDER: S-EPMC1867589 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature