Temporal and spatial patterns of expression of inhibitors of apoptosis in human placentas.

ABSTRACT: The apoptosis cascade that plays a central role in normal and pathological processes is strictly controlled, in part by newly described members of the inhibitor of apoptosis (IAP) family (HIAP-1, HIAP-2, XIAP, NAIP, Survivin, and Livin). Here, we report the expression of IAP mRNAs and proteins in early and late gestation human placentas, term cytotrophoblast cells, and two choriocarcinoma cell lines, JEG-3 and Jar. Reverse transcriptase-polymerase chain reaction identified mRNAs derived from all of the currently known IAP genes in all samples. Analysis by immunoblotting revealed that IAP proteins are present in early and late gestation human placentas and that levels of IAPs are not identical in normal and transformed trophoblast cells. Immunohistochemical experiments performed on paraform

SUBMITTER: Ka H 

PROVIDER: S-EPMC1868194 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature