A highly divergent microsatellite facilitating fast and accurate DRB haplotyping in humans and rhesus macaques.

ABSTRACT: The DRB region of the MHC in primate species is known to display abundant region configuration polymorphism with regard to the number and content of genes present per haplotype. Furthermore, depending on the species studied, the different DRB genes themselves may display varying degrees of allelic polymorphism. Because of this combination of diversity (differential gene number) and polymorphism (allelic variation), molecular typing methods for the primate DRB region are cumbersome. All intact DRB genes present in humans and rhesus macaques appear to possess, however, a complex and highly divergent microsatellite. Microsatellite analysis of a sizeable panel of outbred rhesus macaques, covering most of the known Mamu-DRB haplotypes, resulted in the definition of unique genotyping patterns th

SUBMITTER: Doxiadis GG 

PROVIDER: S-EPMC1868589 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature