Molecular analysis of core kinetochore composition and assembly in Drosophila melanogaster.

ABSTRACT:

Background

Kinetochores are large multiprotein complexes indispensable for proper chromosome segregation. Although Drosophila is a classical model organism for studies of chromosome segregation, little is known about the organization of its kinetochores.

Methodology/principal findings

We employed bioinformatics, proteomics and cell biology methods to identify and analyze the interaction network of Drosophila kinetochore proteins. We have shown that three Drosophila proteins highly diverged from human and yeast Ndc80, Nuf2 and Mis12 are indeed their orthologues. Affinity purification of these proteins from cultured Drosophila cells identified a further five interacting proteins with weak similarity to subunits of the SPC105/KNL-1, MIND/MIS12 and NDC80 kinetochore complexes to

SUBMITTER: Przewloka MR 

PROVIDER: S-EPMC1868777 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature