Proteome characterization of a human urothelial cell line resistant to the bladder carcinogen 4-aminobiphenyl.

ABSTRACT:

Background

The aromatic amine 4-aminobiphenyl (4-ABP) is an environmental and occupational contaminant known to be a major etiological agent of human bladder cancer. 4-ABP metabolites are able to form DNA adducts that may induce mutations and initiate bladder carcinogenesis. Cells exposed to 4-ABP may develop resistance to the carcinogen. The aim of the present study was to detect and identify proteins whose expression is altered in the bladder carcinoma RT112 sub-lines selected for acquired resistance to 4-ABP, in order to disentangle the mechanisms.

Results

Differential proteome analysis of cell lysates showed an overall perturbation in cell metabolism and energy pathways in the 4-ABP-resistant human urothelial clones, with over-expression of membrane trafficking proteins

SUBMITTER: Pastorelli R 

PROVIDER: S-EPMC1871571 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature