Requirement of aggregation propensity of Alzheimer amyloid peptides for neuronal cell surface binding.

ABSTRACT:

Background

Aggregation of the amyloid peptides, Abeta40 and Abeta42, is known to be involved in the pathology of Alzheimer's disease (AD). Here we investigate the relationship between peptide aggregation and cell surface binding of three forms of Abeta (Abeta40, Abeta42, and an Abeta mutant).

Results

Using confocal microscopy and flow cytometry with fluorescently labelled Abeta, we demonstrate a correlation between the aggregation propensity of the Alzheimer amyloid peptides and their neuronal cell surface association. We find that the highly aggregation prone Abeta42 associates with the surface of neuronal cells within one hour, while the less aggregation prone Abeta40 associates over 24 hours. We show that a double mutation in Abeta42 that reduces its aggregation propensit

SUBMITTER: Bateman DA 

PROVIDER: S-EPMC1871596 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature