Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation, and male infertility.

ABSTRACT: We identified a previously uncharacterized gene, spermatid maturation 1 (Spem1), encoding a protein exclusively expressed in the cytoplasm of steps 14-16 elongated spermatids in the mouse testis. This protein contains no known functional domains and is highly conserved across mammalian species. Male mice deficient in Spem1 were completely infertile because of deformed sperm characterized by a bent head wrapped around by the neck and the middle piece of the tail. We show that lack of Spem1 causes failure of the cytoplasm to become loose and detach from the head and the neck region of the developing spermatozoa. Retained cytoplasmic components mechanically obstruct the straightening of the sperm head and the stretching of the growing tail, leading to the bending of the head in the neck, foll

SUBMITTER: Zheng H 

PROVIDER: S-EPMC1871874 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature