The second AT-hook of the architectural transcription factor HMGA2 is determinant for nuclear localization and function.

ABSTRACT: High Mobility Group A (HMGA) is a family of architectural nuclear factors which play an important role in neoplastic transformation. HMGA proteins are multifunctional factors that associate both with DNA and nuclear proteins that have been involved in several nuclear processes including transcription. HMGA localization is exclusively nuclear but, to date, the mechanism of nuclear import for these proteins remains unknown. Here, we report the identification and characterization of a nuclear localization signal (NLS) for HMGA2, a member of the HMGA family. The NLS overlaps with the second of the three AT-hooks, the DNA-binding domains characteristic for this group of proteins. The functionality of this NLS was demonstrated by its ability to target a heterologous beta-galactosidase/green fluo

SUBMITTER: Cattaruzzi G 

PROVIDER: S-EPMC1874589 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature