Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs.

ABSTRACT: Large proteins have multiple domains that are potentially capable of binding many kinds of partners. It is conceivable, therefore, that such proteins could function as an intricate framework of assembly protein complexes. To comprehensively study protein-protein interactions between large KIAA proteins, we have constructed a library composed of 1087 KIAA cDNA clones based on prior functional classifications done in silico. We were guided by two principles that raise the success rate for detecting interactions per tested combination: we avoided testing low-probability combinations, and reduced the number of potential false negatives that arise from the fact that large proteins cannot reliably be expressed in yeast. The latter was addressed by constructing a cDNA library comprised of random

SUBMITTER: Nakayama M 

PROVIDER: S-EPMC187542 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature