Development of expression vectors for Escherichia coli based on the pCR2 replicon.

ABSTRACT:

Background

Recent developments in metabolic engineering and the need for expanded compatibility required for co-expression studies, underscore the importance of developing new plasmid vectors with properties such as stability and compatibility.

Results

We utilized the pCR2 replicon of Corynebacterium renale, which harbours multiple plasmids, for constructing a range of expression vectors. Different antibiotic-resistance markers were introduced and the vectors were found to be 100% stable over a large number of generations in the absence of selection pressure. Compatibility of this plasmid was studied with different Escherichia coli plasmid replicons viz. pMB1 and p15A. It was observed that pCR2 was able to coexist with these E. coli plasmids for 60 generations in the absence

SUBMITTER: Walia R 

PROVIDER: S-EPMC1876245 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature