Expression of stem cells markers in ocular hemangioblastoma associated with von Hippel-Lindau (VHL) disease.

ABSTRACT:

Purpose

To better understand the histogenesis of ocular hemangioblastomas associated with von Hippel-Lindau (VHL) disease.

Methods

We found that co-expression of Epo and EpoR may mediate developmental stagnation and induce proliferation of hemangioblastoma. All lesions were frozen and/or fixed in formalin and embedded in paraffin. The specimens were sectioned and subjected to routine histology, immunohistochemistry and molecular analyses. Avidin-biotin-complex immunoperoxidase was used to evaluate the expression of erythropoietin (Epo), Epo receptor (EpoR), CD31, CD34, CD117, and CD133. Ocular hemangioblastoma cells were microdissected in order to determine expression of Epo and EpoR transcripts using reverse transcription-polymerase chain reaction.

Results

Tumorlet-l

SUBMITTER: Chan CC 

PROVIDER: S-EPMC1876780 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature