Global surveillance of emerging Influenza virus genotypes by mass spectrometry.

ABSTRACT:

Background

Effective influenza surveillance requires new methods capable of rapid and inexpensive genomic analysis of evolving viral species for pandemic preparedness, to understand the evolution of circulating viral species, and for vaccine strain selection. We have developed one such approach based on previously described broad-range reverse transcription PCR/electrospray ionization mass spectrometry (RT-PCR/ESI-MS) technology.

Methods and principal findings

Analysis of base compositions of RT-PCR amplicons from influenza core gene segments (PB1, PB2, PA, M, NS, NP) are used to provide sub-species identification and infer influenza virus H and N subtypes. Using this approach, we detected and correctly identified 92 mammalian and avian influenza isolates, representing 30 di

SUBMITTER: Sampath R 

PROVIDER: S-EPMC1876795 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature