Regulation of cell diameter, For3p localization, and cell symmetry by fission yeast Rho-GAP Rga4p.

ABSTRACT: Control of cellular dimensions and cell symmetry are critical for development and differentiation. Here we provide evidence that the putative Rho-GAP Rga4p of Schizosaccharomyces pombe controls cellular dimensions. rga4 Delta cells are wider in diameter and shorter in length, whereas Rga4p overexpression leads to reduced diameter of the growing cell tip. Consistent with a negative role in cell growth control, Rga4p protein localizes to the cell sides in a "corset" pattern, and to the nongrowing cell tips. Additionally, rga4 Delta cells show an altered growth pattern similar to that observed in mutants of the formin homology protein For3p. Consistent with these observations, Rga4p is required for normal localization of For3p and for normal distribution of the actin cytoskeleton. We show tha

SUBMITTER: Das M 

PROVIDER: S-EPMC1877093 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature