Genome-wide identification of alternatively spliced mRNA targets of specific RNA-binding proteins.

ABSTRACT:

Background

Alternative splicing plays an important role in generating molecular and functional diversity in multi-cellular organisms. RNA binding proteins play crucial roles in modulating splice site choice. The majority of known binding sites for regulatory proteins are short, degenerate consensus sequences that occur frequently throughout the genome. This poses an important challenge to distinguish between functionally relevant sequences and a vast array of those occurring by chance.

Methodology/principal findings

Here we have used a computational approach that combines a series of biological constraints to identify uridine-rich sequence motifs that are present within relevant biological contexts and thus are potential targets of the Drosophila master sex-switch protein Se

SUBMITTER: Robida MD 

PROVIDER: S-EPMC1885218 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature