Temporal regulation of viral transcription during development of Thermus thermophilus bacteriophage phiYS40.

ABSTRACT: Regulation of gene expression of lytic bacteriophage varphiYS40 that infects the thermophilic bacterium Thermus thermophilus was investigated and three temporal classes of phage genes, early, middle, and late, were revealed. varphiYS40 does not encode a (RNAP) and must rely on host RNAP for transcription of its genes. Bioinformatic analysis using a model of Thermus promoters predicted 43 putative sigma(A)-dependent -10/-35 class phage promoters. A randomly chosen subset of those promoters was shown to be functional in vivo and in vitro and to belong to the early temporal class. Macroarray analysis, primer extension, and bioinformatic predictions identified 36 viral middle and late promoters. These promoters have a single common consensus element, which resembles host sigma(A) RNAP holoenzy

SUBMITTER: Sevostyanova A 

PROVIDER: S-EPMC1885378 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature