Sequencing and association analysis of the type 1 diabetes-linked region on chromosome 10p12-q11.

ABSTRACT:

Background

In an effort to locate susceptibility genes for type 1 diabetes (T1D) several genome-wide linkage scans have been undertaken. A chromosomal region designated IDDM10 retained genome-wide significance in a combined analysis of the main linkage scans. Here, we studied sequence polymorphisms in 23 Mb on chromosome 10p12-q11, including the putative IDDM10 region, to identify genes associated with T1D.

Results

Initially, we resequenced the functional candidate genes, CREM and SDF1, located in this region, genotyped 13 tag single nucleotide polymorphisms (SNPs) and found no association with T1D. We then undertook analysis of the whole 23 Mb region. We constructed and sequenced a contig tile path from two bacterial artificial clone libraries. By comparison with a clone li

SUBMITTER: Nejentsev S 

PROVIDER: S-EPMC1885446 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature