The mRNA of L-type calcium channel elevated in colon cancer: protein distribution in normal and cancerous colon.

ABSTRACT: Previous reports indicate that the mRNA for the cardiac isoform of the voltage-gated L-type calcium channel (alpha(1C)) is elevated in colon cancer. The aim of these experiments was to verify that the mRNA for alpha(1C) was significantly increased in tumors of two separate populations of patients when compared to normal adjacent mucosa. The second aim was to measure the distribution of alpha(1C) using immunocytochemistry in normal human colon and in colon cancer and to determine what might regulate the channel expression. Biopsies were taken from patients with various stages of colon cancer and nearby normal mucosa were used as control. RNA was prepared and mRNA level measured by semiquantitative reverse transcriptase-polymerase chain reaction. The mRNA of the calcium channel was compared

SUBMITTER: Wang XT 

PROVIDER: S-EPMC1885745 | biostudies-literature | 2000 Nov

REPOSITORIES: biostudies-literature