Cross platform microarray analysis for robust identification of differentially expressed genes.

ABSTRACT:

Background

Microarrays have been widely used for the analysis of gene expression and several commercial platforms are available. The combined use of multiple platforms can overcome the inherent biases of each approach, and may represent an alternative that is complementary to RT-PCR for identification of the more robust changes in gene expression profiles. In this paper, we combined statistical and functional analysis for the cross platform validation of two oligonucleotide-based technologies, Affymetrix (AFFX) and Applied Biosystems (ABI), and for the identification of differentially expressed genes.

Results

In this study, we analysed differentially expressed genes after treatment of an ovarian carcinoma cell line with a cell cycle inhibitor. Treated versus control RNA was

SUBMITTER: Bosotti R 

PROVIDER: S-EPMC1885857 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature