Analysis of nucleolar protein dynamics reveals the nuclear degradation of ribosomal proteins.

ABSTRACT:

Background

The nucleolus is a subnuclear organelle in which rRNAs are transcribed, processed, and assembled with ribosomal proteins into ribosome subunits. Mass spectrometry combined with pulsed incorporation of stable isotopes of arginine and lysine was used to perform a quantitative and unbiased global analysis of the rates at which newly synthesized, endogenous proteins appear within mammalian nucleoli.

Results

Newly synthesized ribosomal proteins accumulated in nucleoli more quickly than other nucleolar components. Studies involving time-lapse fluorescence microscopy of stable HeLa cell lines expressing fluorescent-protein-tagged nucleolar factors also showed that ribosomal proteins accumulate more quickly than other components. Photobleaching and mass-spectrometry exper

SUBMITTER: Lam YW 

PROVIDER: S-EPMC1885954 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature