Overexpressing centriole-replication proteins in vivo induces centriole overduplication and de novo formation.

ABSTRACT:

Background

Centrosomes have important roles in many aspects of cell organization, and aberrations in their number and function are associated with various diseases, including cancer. Centrosomes consist of a pair of centrioles surrounded by a pericentriolar matrix (PCM), and their replication is tightly regulated. Here, we investigate the effects of overexpressing the three proteins known to be required for centriole replication in Drosophila-DSas-6, DSas-4, and Sak.

Results

By directly observing centriole replication in living Drosophila embryos, we show that the overexpression of GFP-DSas-6 can drive extra rounds of centriole replication within a single cell cycle. Extra centriole-like structures also accumulate in brain cells that overexpress either GFP-DSas-6 or GFP-Sak,

SUBMITTER: Peel N 

PROVIDER: S-EPMC1885955 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature