Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.

ABSTRACT: Genomic array technologies provide a means for profiling global changes in gene expression under a variety of conditions. However, it has been difficult to assess whether transcriptional or posttranscriptional regulation is responsible for these changes. Additionally, fluctuations in gene expression in a single cell type within a complex tissue like a tumor may be masked by overlapping profiles of all cell types in the population. In this paper, we describe the use of cDNA arrays to identify subsets of mRNAs contained in endogenous messenger ribonucleoprotein complexes (mRNPs) that are cell type specific. We identified mRNA subsets from P19 embryonal carcinoma stem cells by using mRNA-binding proteins HuB, eIF-4E, and PABP that are known to play a role in translation. The mRNA profiles ass

SUBMITTER: Tenenbaum SA 

PROVIDER: S-EPMC18875 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature